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Developmental Biology
/
使用体内和组织和细胞
外
植体方法研究胚胎和围产期主动脉的形态和发病机制
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Using
In Vivo
and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
Please note that all translations are automatically generated.
Click here for the English version.
使用体内和组织和细胞
外
植体方法研究胚胎和围产期主动脉的形态和发病机制
DOI:
10.3791/56039-v
•
10:57 min
•
September 12, 2017
•
Ashish Misra
,
Zhonghui Feng
,
Jiasheng Zhang
,
Zhi-Yin Lou
3
,
Daniel M. Greif
1
Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine
,
Yale University School of Medicine
,
2
Department of Neurology
,
Yale University School of Medicine
,
3
Department of Neurology, Xinhua Hospital
,
Shanghai Jiaotong University School of Medicine
Chapters
00:05
Title
01:07
In Vivo
Embryonic Fate Mapping and Clonal Analysis
04:14
Explant Embryonic Aorta Culture
05:47
Smooth Muscle Cell (SMC) Perinatal Aorta Isolation
07:16
Subcutaneous Osmotic Mini-Pump Placement
08:23
Results: Representative Embryonic and Neonatal SMC Analyses
09:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
用于研究胚胎和围产期小鼠主动脉的协议使用
在体内
克隆分析和命运图, 主动脉外植体和孤立平滑肌细胞在这里详述。这些不同的方法有助于调查胚胎和围产期主动脉在正常发育和疾病的发病机制。
Tags
Aorta
Vascular Development
Smooth Muscle
Supravalvular Aortic Stenosis
Embryonic
Perinatal
Morphogenesis
Pathogenesis
In Vivo
Cell Explant
Cre ER
Tamoxifen
Progesterone
Paraformaldehyde
Sucrose
OCT Compound
Cryosectioning
Article
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