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Determination of the Glycogen Content in Cyanobacteria
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Biochemistry
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JoVE Journal Biochemistry
Determination of the Glycogen Content in Cyanobacteria

Determination of the Glycogen Content in Cyanobacteria

DOI:
10.3791/56068-v

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07:04 min

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July 17, 2017

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, ,
1Carlsberg Research Laboratory, 2Department of Biology,University of Copenhagen, 3Copenhagen Plant Science Center, Department of Plant and Environmental Sciences,University of Copenhagen

Chapters

  • 00:05Title
  • 01:01Lysis of Cyanobacterial Cells
  • 02:41Glycogen Precipitation, Enzymatic Hydrolysis, and Glycogen Determination
  • 04:30Determination of Total Glucose Content by the Glucose Oxidase-peroxidase (GOD-POD) Reagent
  • 05:30Results: Measurement of Glycogen Contents in Synechocystis sp.
  • 06:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.

Tags

Keywords: CyanobacteriaGlycogen ContentEnzyme-based AssayMicroorganismsCell LysisProtein ConcentrationChlorophyll A ExtractionEnzyme HydrolysisSpectrophotometric Analysis

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