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Journal
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Biochemistry
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测定蓝细菌中的糖原含量
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Determination of the Glycogen Content in Cyanobacteria
Please note that all translations are automatically generated.
Click here for the English version.
测定蓝细菌中的糖原含量
DOI:
10.3791/56068-v
•
07:04 min
•
July 17, 2017
•
Alice De Porcellinis
,
Niels-Ulrik Frigaard
,
Yumiko Sakuragi
1
Carlsberg Research Laboratory
,
2
Department of Biology
,
University of Copenhagen
,
3
Copenhagen Plant Science Center, Department of Plant and Environmental Sciences
,
University of Copenhagen
Chapters
00:05
Title
01:01
Lysis of Cyanobacterial Cells
02:41
Glycogen Precipitation, Enzymatic Hydrolysis, and Glycogen Determination
04:30
Determination of Total Glucose Content by the Glucose Oxidase-peroxidase (GOD-POD) Reagent
05:30
Results: Measurement of Glycogen Contents in
Synechocystis sp.
06:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个可靠和容易的测定来测量蓝细菌细胞中的糖原含量。该过程需要沉淀,可选择解聚和葡萄糖残留的检测。该方法适用于野生型和遗传工程菌株,可促进蓝细菌的代谢工程。
Tags
Cyanobacteria
Glycogen Content
Enzyme-based Assay
Microorganisms
Cell Lysis
Protein Concentration
Chlorophyll A Extraction
Enzyme Hydrolysis
Spectrophotometric Analysis
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