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纳米颗粒对荷瘤小鼠药相结合的样品提取及同时色谱定量分析
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Cancer Research
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JoVE Journal Cancer Research
Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
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纳米颗粒对荷瘤小鼠药相结合的样品提取及同时色谱定量分析

DOI:
10.3791/56159-v

•

08:57 min

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October 05, 2017

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, , , , , , ,
1Department of Pharmaceutical Sciences,University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute,University Health Network

Chapters

  • 00:05Title
  • 01:20Biological Sample Preparation
  • 03:35HPLC Instrumentation and Operation Parameters
  • 04:50Analysis of Samples
  • 05:46Results: Drug Combination Delivery of Doxorubicin and Mitomycin C in Nanoparticles
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一个高效和方便的分析过程中的样品提取和同时测定多药, 阿霉素 (DOX), 丝裂霉 C (MMC) 和心脏毒性 DOX 代谢物, doxorubicinol (DOXol), 在生物样品的临床前乳腺癌模型治疗的纳米颗粒制剂的协同药物组合。

Tags

DoxorubicinMitomycin CDoxorubicinolNanoparticlesTumorHPLCSimultaneous QuantitationSample PreparationExtractionInternal Standard

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