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Developmental Biology
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通过子代检测改变斑马鱼致死性骨骼突变显
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Developmental Biology
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JoVE Journal
Developmental Biology
Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
Please note that all translations are automatically generated.
Click here for the English version.
通过子代检测改变斑马鱼致死性骨骼突变显
DOI:
10.3791/56200-v
•
08:39 min
•
September 01, 2017
•
Elliott P. Brooks
,
James T. Nichols
1
Department of Craniofacial Biology
,
University of Colorado Anschutz Medical Campus
Chapters
00:05
Title
01:05
KASP Genotyping
03:18
Pairwise Cross Sibling Heterozygous and Embryo Rearing
04:13
Alcian Blue/Alizarin Red Cartilage and Bone Staining
05:42
Phenotype Scoring and Family Selection
06:49
Results: Selective Breeding for Skeletal Penetrance in Zebrafish
07:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
本议定书的目的是改变显致命的骨骼突变体表型在斑马鱼的选择性育种。致命的突变体不能成长为成年和繁殖, 因此本协议描述了通过后代测试来跟踪和选择显的方法。
Tags
Zebrafish
Lethal Skeletal Mutant
Penetrance
Progeny Testing
Selective Breeding
Recessive Lethal
KASP Genotyping
Genomic DNA
PCR
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