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Biochemistry
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用高光谱共聚焦显微镜和荧光寿命成像对发光共轭吩染色的淀粉样组织的成像
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
Please note that all translations are automatically generated.
Click here for the English version.
用高光谱共聚焦显微镜和荧光寿命成像对发光共轭吩染色的淀粉样组织的成像
DOI:
10.3791/56279-v
•
10:04 min
•
October 20, 2017
•
Sofie Nyström
,
Marcus Bäck
,
K. Peter R. Nilsson
,
Per Hammarström
1
IFM-Department of Chemistry
,
Linköping University
Chapters
00:05
Title
01:08
hFTAA Solution and Tissue Preparation
02:57
Hyperspectral Imaging
05:05
Confocal Microscopy
06:44
Fluorescence Lifetime Imaging
07:33
Results: Tissue and Protein Aggregates after h-FTAA Staining
09:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
淀粉样蛋白沉积是不同疾病的标志, 并影响许多不同的器官。本文介绍了荧光结合吩荧光染色技术在光学显微镜下的应用。这种染色方法是一种强有力的工具, 用于检测和探索蛋白质集在临床和科学的设置。
Tags
Amyloid
Protein Aggregates
Prion Disease
Alzheimer’s Disease
Luminescent Conjugated Oligothiophenes
HFTAA
Hyperspectral Confocal Microscopy
Fluorescence Lifetime Imaging
Protein Misfolding Diseases
Microscopy Techniques
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