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量化的信息编码的基因表达水平在寿命调制下线虫的宽范围饮食限制

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

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量化的信息编码的基因表达水平在寿命调制下线虫的宽范围饮食限制

DOI:

10.3791/56292-v

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09:23 min

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August 16, 2017

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Dhaval S Patel*¹, Giovanni Diana*¹, Eugeni V. Entchev, Mei Zhan^3,4, Hang Lu^3,4, QueeLim Ch’ng

¹Centre for Developmental Neurobiology,King’s College London, ²Interdisciplinary Bioengineering Graduate Program,Georgia Institute of Technology, ³Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology, ⁴School of Chemical & Biomolecular Engineering,Georgia Institute of Technology

Chapters

00:05Title
00:54Preparation of Bacterial Cultures and Dilution for Experiments
03:05Treatment of Reporter Strains with egg-5 RNAi
04:55Initiation of Broad-range DR
07:19Results: Information in C. elegans Quantified During Lifespan Modulation Through Broad-range Dietary Restriction
08:44Conclusion

Summary

Automatic Translation

在这里，我们提出一个框架，以宽范围饮食限制涉及基因表达和使用寿命。我们描述协议为宽范围限制饮食和定量成像的这个范式下的基因表达。我们进一步列出了计算分析，以揭示潜在的信息处理功能的基因电路参与食品遥感。

Tags

Keywords: Gene Expression Lifespan Modulation Dietary Restriction C. Elegans Environmental Information Neurobiology Of Aging OP50 LB Medium Spectrophotometer OD600

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