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Journal
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Immunology and Infection
/
靶向半胱氨酸硫用于
体外
重组蛋白的特异糖基化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Targeting Cysteine Thiols for
in Vitro
Site-specific Glycosylation of Recombinant Proteins
Please note that all translations are automatically generated.
Click here for the English version.
靶向半胱氨酸硫用于
体外
重组蛋白的特异糖基化
DOI:
10.3791/56302-v
•
11:25 min
•
October 04, 2017
•
Yoo Jung Choi*
1
,
Jinhui Zhu*
1
,
Steve Chung*
1
,
Naveed Siddiqui*
1
,
Qingping Feng
,
Peter B. Stathopulos
1
Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry
,
University of Western Ontario
Chapters
00:05
Title
01:09
Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis
03:44
Uniform
15
N-labeled Protein Expression in BL21 ΔE3
E. coli
05:24
Purification of Recombinant Protein from
E. coli
07:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
08:48
Results: Site-selective Cys Mutation Followed by
In Vitro
Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
糖化蛋白的生物化学和结构分析需要相对较大数量的均质样品。在这里, 我们提出了一种有效的化学方法的 site-specific 糖基化的重组蛋白纯化细菌的靶向活性胱氨酸硫。
Tags
Cysteine Thiols
Site-specific Glycosylation
Recombinant Proteins
Structural Biology
Glycosylation Patterns
Disease Therapy
PCR Mutagenesis
DPN1 Digestion
Protein Expression
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