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Dendrimer-baserede ujævn Nanopatterns til lokalt kontrol flade Adhesiveness: en metode til direkte Chondrogenic differentiering
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
DOI:

14:46 min

January 20, 2018

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Chapters

  • 00:00Title
  • 01:12Production of Nanopatterned Growth Substrates
  • 05:59Local RGD Surface Density Characterization with AFM
  • 07:49Cell Culture and Induction of Chondrogenesis
  • 08:49Cell Fixation and Immunostaining
  • 10:18Cell Imaging and Data Analysis
  • 12:28Representative Results
  • 13:29Conclusion

Summary

Automatic Translation

En metode til at opnå dendrimer-baserede ujævn nanopatterns, der tillader nanoskala kontrol af lokale arginin-glycin-aspartinsyre-syre (M.H.T.) overflade tæthed er beskrevet og anvendes til undersøgelse af adhæsion og chondrogenic Celledifferentiering.

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