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Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

DOI:
10.3791/56352-v

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10:06 min

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August 03, 2017

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, , ,
1Graduate School of Medical Sciences,Kanazawa University

Chapters

  • 00:05Title
  • 00:58Stage 16 Embryo Collection
  • 02:39Embryonic Preparation
  • 04:50Hemocyte and TUNEL Staining
  • 07:41Results: Representative Hemocyte and Apoptotic Cell Staining
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells.

Tags

Keywords: PhagocytosisApoptotic CellsDrosophila EmbryosImmune ResponseDevelopmental BiologyCell EngulfmentInflammatory DisordersAutoimmune DiseaseCell IsolationCollagenaseCell SuspensionCell HomogenizationCell Trituration

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