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Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

DOI:
10.3791/59088-v

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11:20 min

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March 02, 2019

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, , ,
1Department of Physiological Sciences,Eastern Virginia Medical School

Chapters

  • 00:04Title
  • 00:43Fluorescent Bead Phagocytosis
  • 03:45Fcgamma Receptor (FcgammaR)- and Complement Receptor (CR)-Mediated Phagocytosis
  • 05:33Pattern Recognition Receptor (PRR)-Mediated Phagocytosis
  • 06:41Alveolar Macrophage In Vivo Phagocytosis
  • 08:15In Vivo Bacteria Clearance
  • 09:21Results: Representative Mouse Alveolar Macrophage Phagocytosis and Bacteria Clearance
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here we report common methods to analyze the phagocytic function of murine alveolar macrophages and bacterial clearance from the lung. These methods study in vitro phagocytosis of fluorescein isothiocyanate beads and in vivo phagocytosis of Pseudomonas aeruginosa Green Fluorescent Protein. We also describe a method for clearing P. aeruginosa in mice.

Tags

Keywords: Alveolar MacrophagePhagocytosisPseudomonas AeruginosaBSL-2Intratracheal DeliveryBronchoalveolar LavageBALFPrimary Cell IsolationIn Vitro PhagocytosisIn Vivo PhagocytosisBacterial Clearance

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