Journal
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Immunology and Infection
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Rensning af Viral DNA til identifikation af tilknyttede Viral og cellulær proteiner
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Rensning af Viral DNA til identifikation af tilknyttede Viral og cellulær proteiner

DOI:
10.3791/56374-v

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08:26 min

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August 31, 2017

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,
1Department of Microbiology and Molecular Genetics,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:37Viral Infection and EdC Labeling
  • 01:57Purification of Viral DNA and Associated Proteins
  • 06:21Results: Protein Purification and Proteomics Data
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er specifikt tag og selektivt isolere viral DNA fra inficerede celler til karakterisering af virale genom forbundet proteiner.

Tags

Viral DNA PurificationHerpes Simplex Virus Type 1Proteomic AnalysisCellular FactorsViral GenomeMRC-5 CellsHSV-1 InfectionViral DNA ReplicationEdC LabelingNuclei ExtractionNuclear PelletClick ReactionCellular Proteins

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