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JoVE Journal
Medicine

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At studere hypothalamus Insulin Signal til perifere glukose Intolerance med en løbende Drug Infusion System ind i musen hjernen
 
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At studere hypothalamus Insulin Signal til perifere glukose Intolerance med en løbende Drug Infusion System ind i musen hjernen

Article DOI: 10.3791/56410
January 4th, 2018

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Summary January 4th, 2018

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Denne protokol undersøgelser af chemokine (C-C motiv) ligand 5 (CCL5) i hypothalamus rolle ved at levere en antagonist, MetCCL5, ind i musen hjernen ved hjælp af en mikro-osmotisk pumpe hjernen infusion system. Denne forbigående hæmning af CCL5 aktivitet afbrudt hypothalamus insulin signalering, fører til glukose intolerance og perifere systemisk insulinfølsomhed.

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