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拟南芥种子高通量灭菌的标准化方法
JoVE Journal
Biology
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JoVE Journal Biology
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
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拟南芥种子高通量灭菌的标准化方法

DOI:
10.3791/56587-v

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08:13 min

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October 17, 2017

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, , , ,
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences,The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences,The Ohio State University

Chapters

  • 00:05Title
  • 00:41Sterilization of Arabidopsis Seeds with Bleach
  • 02:14Sterilization of Arabidopsis Seed with Chlorine Gas
  • 05:01Seed Plating and Evaluating Germination
  • 05:59Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

本研究的目的是确定漂白和氯气灭菌对不育培养基上的拟南芥种子萌发的影响。为防止微生物污染物的生长, 在满足种子存活的同时, 制定了优化的灭菌规程。

Tags

Arabidopsis SeedsSeed SterilizationHigh-throughputBleach SolutionTWEEN 20Laminar Flow HoodChlorine GasHydrochloric AcidGermination RateGenomicsProteomicsMetabolomics

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