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Standardiseret metode til høj overførselshastighed Sterilisation af Arabidopsis frø
JoVE Journal
Biology
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JoVE Journal Biology
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardiseret metode til høj overførselshastighed Sterilisation af Arabidopsis frø

DOI:
10.3791/56587-v

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08:13 min

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October 17, 2017

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, , , ,
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences,The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences,The Ohio State University

Chapters

  • 00:05Title
  • 00:41Sterilization of Arabidopsis Seeds with Bleach
  • 02:14Sterilization of Arabidopsis Seed with Chlorine Gas
  • 05:01Seed Plating and Evaluating Germination
  • 05:59Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

Formålet med denne undersøgelse var at fastslå virkningerne af blegemiddel og klor gas sterilisation på frø spiring af en vifte af Arabidopsis genotyper dyrkes på sterile medier. Optimeret sterilisation protokoller er blevet udviklet for at forhindre vækst af mikrobielle forureninger samtidig give tilfredsstillende frø overlevelse.

Tags

Arabidopsis SeedsSeed SterilizationHigh-throughputBleach SolutionTWEEN 20Laminar Flow HoodChlorine GasHydrochloric AcidGermination RateGenomicsProteomicsMetabolomics

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