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Forbedret 3D Hydrogel kulturer af primære Glial celler for In Vitro modellering af Neuroinflammation
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1Department of Psychiatry,University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta, 3Department of Chemical and Materials Engineering,University of Alberta, 4Division of Physical Medicine and Rehabilitation,University of Alberta, 5Centre for Neuroscience,University of Alberta
Chapters
- 00:05Title
- 00:43Coverslip and Mold Preparation
- 02:27Preparation of Cells for Encapsulation
- 04:38Cell Encapsulation
- 06:58Results: Confocal Microscopy and 2D and 3D Mixed Cultures
- 08:47Conclusion
Heri, præsenterer vi en protokol til rotte 3D kultur hjerne-afledte glia celler, herunder astrocytter, mikroglia og oligodendrocytes. Vi demonstrere primære cellekultur, methacrylated hyaluronsyre (HAMA) hydrogel syntese, HAMAphoto-polymerisation og celle indkapsling og prøve behandling for Konfokal og scanning elektron mikroskopisk billeddannelse.
