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Forbedret 3D Hydrogel kulturer af primære Glial celler for In Vitro modellering af Neuroinflammation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
DOI:

09:19 min

December 08, 2017

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Chapters

  • 00:05Title
  • 00:43Coverslip and Mold Preparation
  • 02:27Preparation of Cells for Encapsulation
  • 04:38Cell Encapsulation
  • 06:58Results: Confocal Microscopy and 2D and 3D Mixed Cultures
  • 08:47Conclusion

Summary

Automatic Translation

Heri, præsenterer vi en protokol til rotte 3D kultur hjerne-afledte glia celler, herunder astrocytter, mikroglia og oligodendrocytes. Vi demonstrere primære cellekultur, methacrylated hyaluronsyre (HAMA) hydrogel syntese, HAMAphoto-polymerisation og celle indkapsling og prøve behandling for Konfokal og scanning elektron mikroskopisk billeddannelse.

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