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JoVE Journal Bioengineering
Amélioré l’Hydrogel 3D des Cultures de cellules gliales primaires pour In Vitro la modélisation du neuro-inflammation
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Amélioré l’Hydrogel 3D des Cultures de cellules gliales primaires pour In Vitro la modélisation du neuro-inflammation

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Ici, nous présentons un protocole pour la culture 3D du rat encéphales gliales, y compris les astrocytes, la microglie et les oligodendrocytes. Nous démontrons la culture cellulaire primaire, synthèse de l’acide hyaluronique methacrylated (HAMA) hydrogel, HAMAphoto-polymérisation et le cell encapsulation et traitement d’imagerie microscopique confocal et balayage électronique des échantillons.

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Bioingénierie numéro 130 culture cellulaire 3D hydrogel glia primaire astrocyte microglie oligodendrocytes neuro-inflammation l’acide hyaluronique HAMA
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