7,840 Views
•
10:33 min
•
December 13, 2017
DOI:
Summary
Automatically generated
Genetically encoded luciferase is a popular non-invasive reporter of gene expression. Use of an automated longitudinal luciferase imaging gas- and temperature-optimized recorder (ALLIGATOR) enables longitudinal recording from bioluminescent cells under a wide range of conditions. Here we show how ALLIGATOR may be used in the context of circadian rhythms research.
Related Videos
11:09
Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
Related Videos
39271 Views
10:23
Using Luciferase to Image Bacterial Infections in Mice
Related Videos
20425 Views
07:33
In vivo Dual Substrate Bioluminescent Imaging
Related Videos
15532 Views
05:52
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Related Videos
11623 Views
13:48
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Related Videos
12818 Views
05:06
Bioluminescent Bacterial Imaging In Vivo
Related Videos
15303 Views
10:25
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Related Videos
7578 Views
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Related Videos
22335 Views
04:47
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
Related Videos
10079 Views
05:44
Monitoring Circadian Oscillations with a Bioluminescence Reporter in Organoids
Related Videos
1013 Views
Read Article
Cite this Article
Crosby, P., Hoyle, N. P., O'Neill, J. S. Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR). J. Vis. Exp. (130), e56623, doi:10.3791/56623 (2017).
Copy