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利用自动纵向荧光成像气体和温度优化记录仪 (鳄鱼) 对生物发光记者进行灵活测量
JoVE Journal
Biology
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JoVE Journal
Biology
Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)
Please note that all translations are automatically generated.
Click here for the English version.
利用自动纵向荧光成像气体和温度优化记录仪 (鳄鱼) 对生物发光记者进行灵活测量
DOI:
10.3791/56623-v
•
10:33 min
•
December 13, 2017
•
Priya Crosby
,
Nathaniel P. Hoyle
,
John S. O’Neill
1
MRC Laboratory of Molecular Biology
Chapters
00:05
Title
00:51
Cell Seeding and 3-(N-Morpholino)propanesulfonic Acid (MOPS)-buffered High Glucose Medium Preparation
02:52
Bioluminescent Imaging of Static Tissue Cultures
06:31
Bioluminescent Imaging of Perfused Tissue Cultures
09:19
Results: Bioluminescent Imaging of Mammalian Cells Using an ALLIGATOR
10:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
基因编码荧光是一种受欢迎的非侵入性的转基因记者。使用自动纵向荧光成像气体和温度优化记录仪 (鳄鱼), 使纵向记录从生物发光细胞在广泛的条件。在这里, 我们展示了如何在生理节律研究的背景下使用鳄鱼。
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Bioluminescent Reporters
Longitudinal Luciferase Imaging
ALLIGATOR
Circadian Rhythm
Cell Culture
Temperature Synchronization
DMEM Medium
PH Optimization
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