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Utreda skadliga effekter av låg trycket Plasma sterilisering på överlevnaden för Bacillus subtilis sporer med Live Cell mikroskopi
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Utreda skadliga effekter av låg trycket Plasma sterilisering på överlevnaden för Bacillus subtilis sporer med Live Cell mikroskopi

Article DOI: 10.3791/56666-v • 10:03 min • November 30th, 2017
November 30th, 2017 •
1, 1,2,3, 2, 4, 2, 4, 3, 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

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Detta protokoll illustrerar de viktig på varandra följande steg krävs för att bedöma relevansen av övervakning vitalitet parametern och processer för DNA-reparationer i återuppliva Bacillus subtilis sporer efter behandling med lågtryck plasma av spårning fluorescens-märkt DNA reparation proteiner via tid-löst konfokalmikroskopi och svepelektronmikroskopi.

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Cellbiologi frågan 129 Plasma sterilisering sanering Bacillus subtilis spore motstånd DNA-reparation levande cell bildbehandling SEM cLSM fluorescensmikroskopi
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