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体内荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

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体内荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测

DOI:

10.3791/56765-v

•

07:44 min

•

July 04, 2018

•

Long Mei², Cheng Zhan, Eric Erquan Zhang

¹PTN Joint Graduate Program, School of Life Sciences,Peking University, ²National Institute of Biological Sciences, Beijing

Chapters

00:04Title
00:37Preparation of Optical Fiber
01:14rAAV Cry1 Reporter Injection
03:21Optic Fiber Insertion into the Adult Mouse SCN
04:05In Vivo Fluorescence Signal Monitoring and Data Collection
05:24Results: Flourescence In Vivo Recording in the SCN Under LD and DD Conditions
06:54Conclusion

Summary

Automatic Translation

这种新开发的荧光技术能够长期监测自由运动小鼠视交叉上核 (SCN) 中昼夜节律基因的转录。

Tags

Keywords: In Vivo Monitoring Circadian Clock Gene Expression Mouse Suprachiasmatic Nucleus Fluorescence Reporters Optical Fiber Recombinant AAV Stereotaxic Injection SCN

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