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体内
荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测
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Neuroscience
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Neuroscience
In Vivo
Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
Please note that all translations are automatically generated.
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体内
荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测
DOI:
10.3791/56765-v
•
07:44 min
•
July 04, 2018
•
Long Mei
2
,
Cheng Zhan
,
Eric Erquan Zhang
1
PTN Joint Graduate Program, School of Life Sciences
,
Peking University
,
2
National Institute of Biological Sciences, Beijing
Chapters
00:04
Title
00:37
Preparation of Optical Fiber
01:14
rAAV
Cry1
Reporter Injection
03:21
Optic Fiber Insertion into the Adult Mouse SCN
04:05
In Vivo
Fluorescence Signal Monitoring and Data Collection
05:24
Results: Flourescence
In Vivo
Recording in the SCN Under LD and DD Conditions
06:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
这种新开发的荧光技术能够长期监测自由运动小鼠视交叉上核 (SCN) 中昼夜节律基因的转录。
Tags
In Vivo Monitoring
Circadian Clock Gene Expression
Mouse Suprachiasmatic Nucleus
Fluorescence Reporters
Optical Fiber
Recombinant AAV
Stereotaxic Injection
SCN
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