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Journal
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Neuroscience
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一种改进的麻醉小鼠脑脊液采集方法
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
Please note that all translations are automatically generated.
Click here for the English version.
一种改进的麻醉小鼠脑脊液采集方法
DOI:
10.3791/56774-v
•
06:40 min
•
March 19, 2018
•
Nastasia K-H Lim
2
,
Visse Moestrup
4
,
Xiao Zhang
,
Wen-An Wang
,
Arne Møller
4,6
,
Fu-De Huang
4
1
Shanghai Advanced Research Institute
,
University of Chinese Academy of Sciences, Chinese Academy of Science
,
2
Shanghai Institute of Materia Medica
,
University of Chinese Academy of Sciences, Chinese Academy of Science
,
3
Center of Functionally Integrative Neuroscience, Department of Clinical Medicine
,
Aarhus University
,
4
Sino-Danish Center for Education and Research (SDC)
,
5
Department of Neurology
,
Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine
,
6
Department of Nuclear Medicine and PET-centre
,
Aarhus University Hospital
Chapters
00:05
Title
00:42
Preparation for CSF Collection
01:57
Surgery to Expose the Cisterna Magna
03:34
CSF Collection
05:53
Results: Aβ ELISA Analysis of CSF Sample Collected
06:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了一种改进的技术, 用于丰富的脑脊液 (CSF), 不污染血液。随着样品的收集和纯度的增加, 可以通过脑脊液进行更多的分析, 进一步了解影响大脑和脊髓的疾病。
Tags
Cerebrospinal Fluid
CSF Collection
Anesthetized Mice
Micromanipulator
Pulled Glass Capillary
Capillary Holder
Three-way Valve
Syringe
Surgical Procedure
Stereotaxic Frame
Cisterna Magna
Dura Mater
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