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Un novedoso sistema de alimentador-libre para la producción masiva de células de asesino naturales murinos In Vitro
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
DOI:

10:36 min

January 09, 2018

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Chapters

  • 00:05Title
  • 01:24Preparation of OP9 Conditional Medium
  • 02:55Isolation of Mouse Bone Marrow Cells
  • 06:14siRNA-mediated Gene Silencing of Differentiating Natural Killer Cells
  • 06:43Analysis of Natural Killer Cell Differentiation Using Flow Cytometry
  • 07:35Results: Study of siRNA Dependent Gene Silencing on the Differentiation of Bone Marrow Derived Natural Killer Cells in a Feeder-free In Vitro System
  • 09:27Conclusion

Summary

Automatic Translation

Aquí, presentamos un protocolo para producir en masa las células murinas de silenciamiento génico NK usando un sistema de diferenciación libre de alimentador para estudios mecanísticos in vitro e in vivo.

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