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Journal
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Cancer Research
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otx1 和 otx2 作为鼻窦癌和嗅觉神经母细胞瘤的两种可能的分子标记物的鉴定
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
Please note that all translations are automatically generated.
Click here for the English version.
otx1 和 otx2 作为鼻窦癌和嗅觉神经母细胞瘤的两种可能的分子标记物的鉴定
DOI:
10.3791/56880-v
•
07:00 min
•
February 28, 2019
•
Giovanni Micheloni*
1
,
Giorgia Millefanti*
1
,
Andrea Conti
,
Cristina Pirrone
,
Alessandro Marando
,
Alessia Rainero
,
Lucia Tararà
,
Andrea Pistochini
,
Francesco Lo Curto
,
Francesco Pasquali
,
Paolo Castelnuovo
,
Francesco Acquati
,
Annalisa Grimaldi
,
Roberto Valli
,
Giovanni Porta
1
Department of Medicine and Surgery
,
University of Insubria
,
2
Department of Surgical and Morphological Science
,
University of Insubria
,
3
Department of Biotechnology and Life Science
,
University of Insubria
Chapters
00:04
Title
00:58
Immunochemistry
03:03
Real-time PCR and Data Analysis
04:56
Results: Representative Images of OTX Immunohistochemical Expression in Control and Neoplastic Tissues
05:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
同源基因是调节基因, 通常与成人生物中的肿瘤有关。我们通过免疫组织化学和实时 pcr 分析, 在正常和炎症性鼻黏膜和鼻窦肿瘤中研究了它们的比较表达, 以便将它们作为可能的诊断和治疗靶点。
Tags
OTX1
OTX2
Sinonasal Carcinomas
Olfactory Neuroblastomas
Molecular Markers
RNA Extraction
Real-time PCR
Immunohistochemistry
FFPE Samples
Antigen Retrieval
Triton X
Secondary Antibody
ABC Peroxidase
Diaminobenzene
Hematoxylin
Dehydration
Probe-based PCR
Article
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