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小鼠视网膜色素上皮细胞的原代培养
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Biology
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Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
Please note that all translations are automatically generated.
Click here for the English version.
小鼠视网膜色素上皮细胞的原代培养
DOI:
10.3791/56997-v
•
09:35 min
•
March 16, 2018
•
Peng Shang
,
Nadezda A. Stepicheva
,
Stacey Hose
,
J. Samuel Zigler, Jr.
,
Debasish Sinha
2
1
Department of Ophthalmology
,
University of Pittsburgh School of Medicine
,
2
Wilmer Eye Institute
,
The Johns Hopkins University School of Medicine
Chapters
00:04
Title
00:50
Isolating and Preparing Eyeballs for Dissection
03:18
Isolating the RPE-choroid-sclera Complex from Eyeballs
05:07
Collecting and Culturing Primary RPE Cells from RPE-choroid-sclera Complexes
07:22
Results: Characterize Mouse Primary RPE Cultures
08:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
视网膜色素上皮 (RPE) 是一种多功能的眼睛上皮。在这里, 我们提出了建立从小鼠视网膜色素形成的主要细胞培养的协议。
Tags
Primary Cell Culture
Mouse Retinal Pigment Epithelium
RPE Isolation
RPE Cell Culture
Euthanasia
Eye Dissection
Dispase II
Laminar Flow Cabinet
Growth Medium
Microscopy
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