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Ein Time-Lapse, markierungsfreie, Quantitative Phase bildgebende Untersuchung der ruhenden und aktiven menschlichen Krebszellen
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
DOI:

12:48 min

February 16, 2018

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Chapters

  • 00:04Title
  • 00:58Cell Preparation
  • 02:35Cell Preparation: Splitting KHOS-A and KHOS-N Cells
  • 03:50Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
  • 04:30Quantitative Phase Image Acquisition: Cover Slip Set-up
  • 05:19Quantitative Phase Imaging
  • 07:03Analyzing Cell Morphology
  • 08:20Analyzing Cell Proliferation
  • 08:54Analyzing Cell Motion
  • 10:16Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
  • 11:50Conclusion

Summary

Automatic Translation

Ruhende und aktive Krebs Zelle Phänotypen zeichneten sich mit quantitativen Phase Bildgebung. Cell Proliferation, Migration und Morphologie-Assays wurden integriert und in einer einfachen Methode analysiert.

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