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Journal
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Cancer Research
/
休眠和活性人肿瘤细胞的时间推移、无标签、定量相成像研究
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Cancer Research
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JoVE Journal
Cancer Research
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
休眠和活性人肿瘤细胞的时间推移、无标签、定量相成像研究
DOI:
10.3791/57035-v
•
12:48 min
•
February 16, 2018
•
Jing Huang*
1,2
,
Peng Guo*
1,2
,
Marsha A. Moses
2
1
Vascular Biology Program
,
Boston Children’s Hospital
,
2
Department of Surgery
,
Harvard Medical School and Boston Children’s Hospital
Chapters
00:04
Title
00:58
Cell Preparation
02:35
Cell Preparation: Splitting KHOS-A and KHOS-N Cells
03:50
Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
04:30
Quantitative Phase Image Acquisition: Cover Slip Set-up
05:19
Quantitative Phase Imaging
07:03
Analyzing Cell Morphology
08:20
Analyzing Cell Proliferation
08:54
Analyzing Cell Motion
10:16
Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
11:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
应用定量相成像技术对休眠和活性癌细胞表型进行了表征。用一种简单的方法对细胞增殖、迁移和形态学测定进行了综合分析。
Tags
Time-lapse
Label-free
Quantitative Phase Imaging
Dormant Cancer Cells
Active Cancer Cells
Angiogenic Phenotype
Non-angiogenic Phenotype
Osteosarcoma Cells
Cell Morphology
Cell Proliferation
Cell Motility
Tumor Dormancy
Endogenic Switch
Multicellular Parameters
Cell Area
Cell Thickness
Cell Volume
Proliferation Rate
Doubling Time
Motility Speed
Migration
Cell Culture
Cell Passaging
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