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Developmental Biology
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使用磁纳米粒子转导病毒载体进行冷冻切片的原小鼠肠道器官的遗传工程
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
Please note that all translations are automatically generated.
Click here for the English version.
使用磁纳米粒子转导病毒载体进行冷冻切片的原小鼠肠道器官的遗传工程
DOI:
10.3791/57040-v
•
07:37 min
•
May 10, 2019
•
Lingling Xian
,
Lionel Chia
5
,
Dan Georgess
,
Li Luo
,
Shuai Shuai
,
Andrew J. Ewald
4
,
Linda M.S. Resar
4,5,6
1
Department of Medicine, Division of Hematology
,
Johns Hopkins University School of Medicine
,
2
Department of Natural Sciences, School of Arts & Sciences
,
Lebanese American University
,
3
Department of Cell Biology
,
Johns Hopkins University School of Medicine
,
4
Department of Oncology
,
Johns Hopkins University School of Medicine
,
5
Department of Pathology
,
Johns Hopkins University School of Medicine
,
6
Institute for Cell Engineering
,
Johns Hopkins University School of Medicine
Chapters
00:04
Title
01:29
Genetic Engineering of Organoids by Viral Transduction
02:26
Seeding of Infected Organoid Fragments
03:44
Organoid Cryosection in Basement Membrane Matrix
05:32
Results: Efficient Viral Transduction of Intestinal Organoids
06:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了分步说明:1) 使用磁性纳米粒子对慢透或抗逆转录病毒转导进行高效工程肠道有机体,以及 2) 从工程有机物生成冷冻部分。这种方法提供了一个强大的工具,有效地改变基因表达在有机体的研究下游效应。
Tags
Genetic Engineering
Mouse Intestinal Organoids
Magnetic Nanoparticle Transduction
Viral Vectors
Frozen Sectioning
Organoid Cultures
Genetic Manipulation
Intestinal Epithelium
Developmental Processes
Pathologic Processes
Transduction
Basement Matrix
2D Cell Culture
Article
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