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Genteknologi af primære mus intestinale Organoider ved hjælp af magnetisk nanopartikel Transduktionsvirale vektorer for frosne skæring
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1Department of Medicine, Division of Hematology,Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences,Lebanese American University, 3Department of Cell Biology,Johns Hopkins University School of Medicine, 4Department of Oncology,Johns Hopkins University School of Medicine, 5Department of Pathology,Johns Hopkins University School of Medicine, 6Institute for Cell Engineering,Johns Hopkins University School of Medicine
Chapters
- 00:04Title
- 01:29Genetic Engineering of Organoids by Viral Transduction
- 02:26Seeding of Infected Organoid Fragments
- 03:44Organoid Cryosection in Basement Membrane Matrix
- 05:32Results: Efficient Viral Transduction of Intestinal Organoids
- 06:21Conclusion
Vi beskriver trin-for-trin instruktioner til: 1) effektivt ingeniør tarm organoider ved hjælp af magnetiske nanopartikler til Lenti-eller antiretroviral transduktion, og, 2) generere frosne sektioner fra manipuleret organoider. Denne fremgangsmåde giver et effektivt værktøj til effektiv ændring af genekspression i organoider til undersøgelse af downstream-effekter.
