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Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels
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JoVE Journal Neuroscience
Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

DOI:
10.3791/57052-v

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09:55 min

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February 14, 2018

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,
1Department of Biology,University of Maryland

Chapters

  • 00:04Title
  • 00:42Preparation
  • 03:17Obtaining a Whole-cell Recording Configuration
  • 05:36Testing for Synaptic Connectivity
  • 07:17Results: Using Tetrotoxin Engineered Resistance for Probing Synapses (TERPS)
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

This article features a method to test the monosynaptic connections between neurons by employing tetrodotoxin and the tetrodotoxin-resistant sodium channel, NaChBac.

Tags

Keywords: Monosynaptic ConnectionsDrosophilaTetrodotoxin Resistant Sodium ChannelsElectrophysiologyDissectionPatch-clamp RecordingsRecording SalineCsChrimsonAll-trans-RetinalAntennal LobesGlial Sheathing

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