Journal
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Generation af monoklonale antistoffer mod naturprodukter
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Monoclonal Antibodies Against Natural Products
DOI:

12:15 min

April 06, 2019

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Chapters

  • 00:00Title
  • 01:59Preparation of Immunogen and Coating Antigen
  • 02:50Immunity
  • 03:47Preparation of Cell Fusion
  • 05:39Cell Fusion
  • 08:34icELISA
  • 09:19Preparation of Monoclonal Hybridomas
  • 10:06Results
  • 11:53Conclusion

Summary

Automatic Translation

Denne artikel giver en detaljeret protokol for forberedelse og evaluering af monoklonale antistoffer mod naturlige produkter til brug i forskellige immunassays. Denne procedure omfatter vaccination, cellefusion, indirekte konkurrerende ELISA for positive klon screening og monoklonale hybridom forberedelse. Specifikationer for antistof karakterisering ved hjælp af MALDI-TOF-MS og ELISA analyser er også tilgængelige.

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