A subscription to JoVE is required to view this content. Sign in or start your free trial.
1School of Life Science,Beijing University of Chinese Medicine, 2Third Affiliated Hospital,Beijing University of Chinese Medicine, 3School of Basic Medical Sciences,Beijing University of Chinese Medicine, 4School of Chinese Materia Medica,Beijing University of Chinese Medicine, 5Center of Scientific Experiment,Beijing University of Chinese Medicine
Chapters
- 00:00Title
- 01:59Preparation of Immunogen and Coating Antigen
- 02:50Immunity
- 03:47Preparation of Cell Fusion
- 05:39Cell Fusion
- 08:34icELISA
- 09:19Preparation of Monoclonal Hybridomas
- 10:06Results
- 11:53Conclusion
Denne artikkelen inneholder en detaljert protokoll og evaluering av monoklonale antistoffer mot naturlige produkter for bruk i ulike immunanalyser. Denne fremgangsmåten inneholder immunisering, celle fusion, indirekte konkurransedyktig ELISA for positiv klone screening og monoklonalt hybridoma forberedelse. Spesifikasjonene for antistoff karakterisering ved hjelp MALDI-TOF-MULTIPLE Sclerosis og ELISA Analyser tilbys også.
