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针对天然产物的单克隆抗体的产生
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Monoclonal Antibodies Against Natural Products
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针对天然产物的单克隆抗体的产生

DOI:
10.3791/57116-v

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12:15 min

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April 06, 2019

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, , , , , ,
1School of Life Science,Beijing University of Chinese Medicine, 2Third Affiliated Hospital,Beijing University of Chinese Medicine, 3School of Basic Medical Sciences,Beijing University of Chinese Medicine, 4School of Chinese Materia Medica,Beijing University of Chinese Medicine, 5Center of Scientific Experiment,Beijing University of Chinese Medicine

Chapters

  • 00:00Title
  • 01:59Preparation of Immunogen and Coating Antigen
  • 02:50Immunity
  • 03:47Preparation of Cell Fusion
  • 05:39Cell Fusion
  • 08:34icELISA
  • 09:19Preparation of Monoclonal Hybridomas
  • 10:06Results
  • 11:53Conclusion

Summary

Automatic Translation

Automatic Translation

本文提供了一个详细的方案, 用于制备和评价用于各种免疫检测的天然产品单克隆抗体。该程序包括免疫接种、细胞融合、用于阳性克隆筛选的间接竞争性 ELISA 和单克隆杂交瘤制剂。还提供了使用 maldi TOF-MS 和 ELISA 分析进行抗体表征的规范。

Tags

Monoclonal AntibodiesELISANatural ProductsTCMArtificial AntigenHaptenCarrier ProteinMouse ImmunizationCell FusionHybridomaNariginBSA ConjugateFreund’s Adjuvant

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