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Kvantitativ analyse af Neuronal dendritiske Arborization kompleksitet i Drosophila
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Kvantitativ analyse af Neuronal dendritiske Arborization kompleksitet i Drosophila

DOI:
10.3791/57139-v

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07:13 min

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January 07, 2019

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, ,
1Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital,Mass General Institute of Neurodegenerative Disease, 2Department of Geriatric Neurology, Nanlou Clinical Division,PLA General Hospital

Chapters

  • 00:04Title
  • 01:01Larvae Collection and Dissection
  • 02:57Imaging Processing
  • 05:32Results: Silencing Sox102F Affects Dendrite Size
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol fokuserer på kvantitativ analyse af neuronal dendritiske arborization kompleksitet (NDAC) i Drosophila, som kan bruges til undersøgelser af dendritiske morfogenese.

Tags

Dendritic ArborizationDrosophilaSOX5 GeneNeuro-dendritesMorphogenesisNeurodegenerative DiseaseUAS-GFPPpk-GAL4UAS-Sox102F-RNAiThird Instar LarvaeDissectionPBSPFAAntifade Mounting MediumConfocal MicroscopyFiji ImageJDendrite Length

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