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Journal
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Biochemistry
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膜蛋白对洗涤剂敏感相互作用的检测
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Detection of Detergent-sensitive Interactions Between Membrane Proteins
Please note that all translations are automatically generated.
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膜蛋白对洗涤剂敏感相互作用的检测
DOI:
10.3791/57179-v
•
10:09 min
•
March 07, 2018
•
Nava Zaarur
,
Xiang Pan
,
Konstantin V. Kandror
1
Department of Biochemistry
,
Boston University School of Medicine
Chapters
00:04
Title
01:05
Handling of the Peptide and Cells
03:02
Binding of His-tagged Proteins to the HisPur Cobalt Beads
04:39
Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
05:59
Elution from Cobalt Beads
07:07
Electrophoresis, Western Blotting, and Analysis of Results
07:58
Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
09:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个协议, 以检测在膜蛋白之间的洗涤剂敏感的相互作用, 使用结合的排序受体, sortilin, 到第一个腔内循环的葡萄糖转运蛋白, GLUT4, 作为一个例子。
Tags
Membrane Proteins
Detergent-sensitive Interactions
Histidine-tagged
Myc-tagged
Co-immunoprecipitation
Cell Lysate
3T3-L1 Pre-adipocytes
Transfection
Protein Interactions
Biochemistry
Article
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