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Detección de detergente-sensible a las interacciones entre proteínas de la membrana
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Chapters
- 00:04Title
- 01:05Handling of the Peptide and Cells
- 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
- 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
- 05:59Elution from Cobalt Beads
- 07:07Electrophoresis, Western Blotting, and Analysis of Results
- 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
- 09:18Conclusion
Se describe un protocolo para la detección de detergente-sensible a las interacciones entre proteínas de la membrana mediante la Unión del receptor de la clasificación, sortilina, para el primer bucle luminal de la proteína del transportador de glucosa, GLUT4, como ejemplo.
