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用球形发芽法评价 Hemangioblastoma 新生血管的体外性能的综合程序
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
DOI:

08:26 min

April 12, 2018

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Chapters

  • 00:04Title
  • 01:46Cell Culture and Plasmid Construct
  • 03:00Lentivirus Package and Infection
  • 04:19Generation of Modified Endothelial Cell Spheroids
  • 05:04In Vitro Angiogenesis Assay
  • 06:26Results: Use of Spheroid Sprouting Assay to Study Modified Endothelial Cell Vessel Formation
  • 07:30Conclusion

Summary

Automatic Translation

本文提出了一个综合的方法来评价在体外是否存在经典的肿瘤血管生成在小脑 (哈佛商学院) 及其在哈佛商学院的作用。研究结果突出了血红蛋白-新生血管的复杂性, 认为这种共同的血管生成形式仅仅是血红蛋白-新生血管的补充机制。

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