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Bioengineering
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微流控装置中组织模型的 Perfusable 血管网
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
Please note that all translations are automatically generated.
Click here for the English version.
微流控装置中组织模型的 Perfusable 血管网
DOI:
10.3791/57242-v
•
07:05 min
•
April 04, 2018
•
Yuji Nashimoto
,
Yukako Teraoka
,
Ramin Banan Sadeghian
,
Akiko Nakamasu
,
Yuichiro Arima
,
Sanshiro Hanada
,
Hidetoshi Kotera
,
Koichi Nishiyama
,
Takashi Miura
,
Ryuji Yokokawa
1
Department of Micro Engineering
,
Kyoto University
,
2
Graduate School of Medical Sciences
,
Kyushu University
,
3
International Research Center for Medical Sciences (IRCMS)
,
Kumamoto University
Chapters
00:04
Title
00:48
Polydimethylsiloxane (PDMS) Layer Fabrication and Assembly
01:48
Microfluidic Device Cell Seeding
05:12
Results: Representative Vascular Lumen Formation
06:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了如何在球体中设计一个 perfusable 的血管网。球体周围的微环境被设计来诱导血管生成, 并将球体连接到微流控装置中的微。该方法允许球状的灌注, 这是一个期待已久的技术在三维文化。
Tags
Microfluidic Device
Perfusable Vascular Network
Spheroid
HLF
RFP-HUVEC
GFP-HUVEC
Endothelial Medium
Master Mix Solution
Regenerative Medicine
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