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真菌病原体液体荧光恢复法测定脂质指数黑穗病玉米
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
DOI:

10:22 min

April 03, 2018

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Chapters

  • 00:04Title
  • 01:07Culture Condition and Cell Fixation
  • 04:14Liquid Fluorescence Recovery Assay (LFR)
  • 06:13Calculations of the LD Index
  • 07:26Results: LD Index Measurements Enable the Study of TAG Dynamics in U. maydis
  • 08:59Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个获得脂滴指数 (LD 指数) 的协议, 以研究在高通量实验中培养的细胞中甘油三酯的动力学。LD 指数法是一种使用 BODIPY 493/503 的简便可靠的方法。这种检测不需要 dispendious 脂提取或显微分析。

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