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Developmental Biology
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ゼブラフィッシュ胚における細胞光電を介して次の心内膜組織運動
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Developmental Biology
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JoVE Journal
Developmental Biology
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo
Please note that all translations are automatically generated.
Click here for the English version.
ゼブラフィッシュ胚における細胞光電を介して次の心内膜組織運動
DOI:
10.3791/57290-v
•
09:38 min
•
February 20, 2018
•
Renee Wei-Yan Chow*
1,2,3,4
,
Paola Lamperti*
1,2,3,4
,
Emily Steed
2,3,4
,
Francesco Boselli
2,3,4
,
Julien Vermot
2,3,4
1
Institut de Génétique et de Biologie Moléculaire et Cellulaire
,
2
UMR7104
,
Centre National de la Recherche Scientifique
,
3
U964
,
Institut National de la Santé et de la Recherche Médicale
,
4
Université de Strasbourg
Chapters
00:04
Title
00:53
Preparing Molds and Mounting Agarose and Embedding Embryos
03:31
Photoconversion
05:12
Unmounting Photoconverted Embryos
06:20
Imaging Photoconverted Cells at Later Embryonic Stages
08:05
Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
09:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは楓生活の心内膜細胞の蛍光タンパク質の光変換の方法を記述するゼブラフィッシュ胎児房室管、心臓房室弁開発中心内膜細胞の追跡を有効にします。.
Tags
Cell Photoconversion
Zebrafish Embryo
Atrioventricular Canal
Heart Valve Formation
Cardiac Development
Mutants
Time-lapse Microscopy
Agarose
Mounting Medium
Low Melting Point Agarose
Tricaine
BDM
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