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Developmental Biology
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在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后
DOI:
10.3791/57290-v
•
09:38 min
•
February 20, 2018
•
Renee Wei-Yan Chow*
1,2,3,4
,
Paola Lamperti*
1,2,3,4
,
Emily Steed
2,3,4
,
Francesco Boselli
2,3,4
,
Julien Vermot
2,3,4
1
Institut de Génétique et de Biologie Moléculaire et Cellulaire
,
2
UMR7104
,
Centre National de la Recherche Scientifique
,
3
U964
,
Institut National de la Santé et de la Recherche Médicale
,
4
Université de Strasbourg
Chapters
00:04
Title
00:53
Preparing Molds and Mounting Agarose and Embedding Embryos
03:31
Photoconversion
05:12
Unmounting Photoconverted Embryos
06:20
Imaging Photoconverted Cells at Later Embryonic Stages
08:05
Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
09:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了在活斑马鱼胚胎的心内膜细胞中 photoconversion 枫荧光蛋白的方法, 能够在房室管和房室心脏瓣膜发育过程中跟踪心内膜细胞。.
Tags
Cell Photoconversion
Zebrafish Embryo
Atrioventricular Canal
Heart Valve Formation
Cardiac Development
Mutants
Time-lapse Microscopy
Agarose
Mounting Medium
Low Melting Point Agarose
Tricaine
BDM
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