JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
用最小组织量测定果蝇透纤维中线粒体耗氧量
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
Please note that all translations are automatically generated.
Click here for the English version.
用最小组织量测定果蝇透纤维中线粒体耗氧量
DOI:
10.3791/57376-v
•
14:55 min
•
April 07, 2018
•
Chloé J Simard*
1
,
Guillaume Pelletier*
1
,
Luc H Boudreau
,
Etienne Hebert-Chatelain
,
Nicolas Pichaud
1
Département de chimie et biochimie
,
Université de Moncton
,
2
Département de biologie
,
Université de Moncton
Chapters
00:00
Title
00:47
Introduction
01:33
Calibration
03:01
Sample Preparation
05:39
Mitochondrial Oxygen Consumption Experiment
09:31
Representative results
13:57
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了果蝇透胸部高分辨率 respirometry 测量耗氧量的方法。与经典的线粒体分离技术相比, 这种技术需要最少的组织量, 获得的结果与生理相关。
Tags
Drosophila
Mitochondrial Respiration
High-resolution Respirometry
Permeabilized Fibers
Electron Transport System
Substrate Oxidation
Uncoupler
Inhibitors
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em
含黄素线粒体脱氢酶同时测定超氧化物/过氧化氢和 NADH 生产
荷尔斯坦奶牛肝线粒体耗氧量和质子泄漏动力学评价线粒体呼吸
全飞头段耗氧率的测定与解释
使用基于化学探针的免疫测定直接测量KDM1A目标参与
大鼠胰腺组织快速、经济高效的RNA提取
测量来自母猪后代的先前冷冻心脏组织中的线粒体电子转移复合物:评估运动诱导的线粒体生物能量变化的模型
使用透射电子显微镜定量骨骼肌纤维中的亚细胞糖原分布
测量哺乳动物线粒体功能的氧非依赖性测定
基于图像的高通量线粒体DNA合成和分布定量
Read Article