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Lambda 选择cII突变检测系统
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
The Lambda Select cII Mutation Detection System
DOI:

07:08 min

April 26, 2018

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Chapters

  • 00:04Title
  • 00:52In Vitro Packaging Reaction
  • 01:51Plating the Packaged DNA Samples
  • 03:36Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
  • 05:31Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
  • 06:31Conclusion

Summary

Automatic Translation

我们描述了一个详细的协议, 为 Lambda 选择cII突变试验的培养细胞的转基因啮齿动物或相应的动物处理的化学/物理剂的利益。该方法已广泛用于哺乳动物细胞致癌物质的诱变检测。

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