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Immunology and Infection
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Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

DOI:
10.3791/57566-v

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07:24 min

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October 19, 2018

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, , ,
1Institut für Molekulare Zellbiologie

Chapters

  • 00:04Title
  • 01:00Resident Mouse Peritoneal Macrophage and Human Red Blood Cell Isolation
  • 04:00Macrophage Plasma Membrane Labeling and Human Red Blood Cell-mouse Immunoglobulin (IgG) Opsonization and Imaging
  • 06:32Results: Representative Time-lapse, 3D-imaging of Phagocytosis
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.

Tags

Keywords: PhagocytosisMacrophagesTime-lapse3D ImagingCell IsolationPeritoneal LavageRPMI MediumHEPESFibronectin-coated SlidesAir Bubble RemovalCell IncubationMicroscopy

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