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JoVE Journal
Immunology and Infection

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Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
 

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Article doi: 10.3791/59731
May 24th, 2019

Summary May 24th, 2019

Here, we present a protocol to prepare apoptotic thymocytes and peritoneal macrophages and analyze the efficiency of efferocytosis and the specific inhibitor-mediated blocking of apoptotic thymocytes engulfment. This protocol has a broad application in cell-mediated clearance of other particles including artificial beads and bacteria.

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