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Journal
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Biochemistry
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电化学检测氘动力学同位素对
希瓦氏
菌 oneidensis MR-1 细胞外电子传输的影响
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in
Shewanella oneidensis
MR-1
Please note that all translations are automatically generated.
Click here for the English version.
电化学检测氘动力学同位素对
希瓦氏
菌 oneidensis MR-1 细胞外电子传输的影响
DOI:
10.3791/57584-v
•
09:00 min
•
April 16, 2018
•
Yoshihide Tokunou
,
Kazuhito Hashimoto
,
Akihiro Okamoto
1
Department of Applied Chemistry
,
University of Tokyo
,
2
Global Research Center for Environment and Energy based on Nanomaterials Science
,
National Institute for Materials Science
Chapters
00:05
Title
01:00
Formation of a Monolayer Biofilm of
S. oneidensis
MR-1 on an ITO Electrode
04:11
Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate
05:24
Addition of Deuterium Water to Measure the KIE on the EET Process
06:26
Results: Microbial Current Change Induced by D
2
O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process
07:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个全细胞电化学实验的协议, 以研究质子传输的贡献, 通过外膜细胞色素复合体在
希瓦
氏菌 oneidensis MR-1 的细胞外电子转运率。
Tags
Deuterium Kinetic Isotope Effect
Extracellular Electron Transport
Shewanella Oneidensis MR-1
Electrochemical Detection
Proton Transport
C-type Cytochromes
Three-electrode Electrochemical Reactor
ITO Substrate
Anaerobic Conditions
Lactate
Yeast Extract
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