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Elektrokemisk detektion af Deuterium Kinetic isotop effekt på ekstracellulære elektron Transport i Shewanella oneidensis hr.-1
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
DOI:

09:00 min

April 16, 2018

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Chapters

  • 00:05Title
  • 01:00Formation of a Monolayer Biofilm of S. oneidensis MR-1 on an ITO Electrode
  • 04:11Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate
  • 05:24Addition of Deuterium Water to Measure the KIE on the EET Process
  • 06:26Results: Microbial Current Change Induced by D2O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process
  • 07:36Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol af hele-celle elektrokemiske eksperimenter til at studere bidrag af proton transport til sats af ekstracellulære elektron transport via de ydre membran cytokromer, der i Shewanella oneidensis hr.-1.

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