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Journal
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Biochemistry
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用斑点印迹分析 A11-positive β淀粉样寡聚物的制备与评价
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Biochemistry
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JoVE Journal
Biochemistry
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Please note that all translations are automatically generated.
Click here for the English version.
用斑点印迹分析 A11-positive β淀粉样寡聚物的制备与评价
DOI:
10.3791/57592-v
•
06:17 min
•
May 22, 2018
•
Huang Chunhui
2
,
Xu Dilin
,
Zhang Ke
,
Shentu Jieyi
,
Yan Sicheng
,
Wu Dapeng
,
Wang Qinwen
,
Cui Wei
2
1
Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine
,
Ningbo University
,
2
Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center
,
Ningbo University
,
3
School of Materials Science and Chemical Engineering
,
Ningbo University
Chapters
00:05
Title
00:31
Sample Preparation
02:08
Dot Blotting Analysis
03:47
Results: Use of Synthetic Peptides and Dot Blot Analysis to Prepare and Evaluate the Amount of Aβ Oligomers
05:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述如何从合成肽
体外
制备 Aβ寡聚物, 并通过斑点印迹分析来评价 Aβ的相对含量。
Tags
A-beta Oligomer
Dot Blotting Analysis
Curcumin
Anti-oligomer Antibody A11
Anti-A-beta Antibody 6E10
Nitrocellulose Membrane
Bovine Serum Albumin
TBST Buffer
Magnetic Agitator
Centrifugation
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