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Split grønne fluorescerende Protein System å visualisere effektor levert fra bakterier under infeksjon
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JoVE Journal Biology
Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Split grønne fluorescerende Protein System å visualisere effektor levert fra bakterier under infeksjon

DOI:
10.3791/57719-v

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07:25 min

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May 24, 2018

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, , , ,
1Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science,Seoul National University, 2Department of Bioindustry and Bioresource Engineering,Sejong University

Chapters

  • 00:04Title
  • 00:49Preparation of Plant Materials and Pseudomonas Culture
  • 02:09Transient Expression of Organelle-targeted sfGFP1-10OPT in N. benthamiana
  • 03:58Inoculation of Pseudomonas and Observation of sfGFP Signal via Confocal Microscopy
  • 05:46Results: Split Superfolder Fluorescent Protein System: A Useful Tool to Examine the Dynamics of Effectors in Host Plants
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Fluorescerende protein-baserte tilnærminger til overvåke effektor utskilles av bakterier i vertsceller er utfordrende. Dette skyldes inkompatibilitet mellom fluorescerende proteiner og type-III sekresjon systemet. Her, brukes en optimalisert delt superfolder GFP system for visualisering av effektor utskilles av bakterier i verten anlegget celle.

Tags

Split Green Fluorescent ProteinEffectorsPlant-microbe InteractionType III Secretion SystemPseudomonas SyringaeAgrobacterium TumefaciensInfiltrationVisualization

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